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1.1 Delivering Downstream Value: Collaborating for Success in Branded Products The holy grail of renewable chemistry is a consumer or industrial final product. This requires myriad skills in formulation, branding, marketing, channel strategy, logistics, packaging and retail management that are complex, and can create significant barriers to entry. This session will examine how renewable chemical companies are overcoming these challenges through collaboration, a direct investment in developing new skills and a strategic focus on creating and delivering downstream value to the ultimate customer, whether that be retail or industrial. 90 minutes Panel Timothy Staub
Jason Lumsden
Rick Hanson
John Shaw
James Iademarco - Moderator
1.2 Bioeconomy Policy Under Political Climate Change: Will “Made in the USA” Trump Cheap Fossil Fuels? New political directions indicate a likelihood of substantial shifts in US energy policy. In this session, leading energy and financial experts will evaluate the continuing case for biofuels and the bioeconomy, the influence of policy on bioeconomy investments, whether “exernality” benefits can be meaningfully captured in market pricing mechanisms, the ongoing viability of purchase requirements and policy options going forward, including how potential cost and the near-term prospects impact the bottom line. 75 Minutes Panel Vineet Rajgarhia
Jim Lane
Paul Bryan
Peter Matlock - Moderator
1.3 Global Knowledge Development with International Partners The world is getting smaller with our ability to communicate instantly and to travel the globe efficiently. That and the fact that Innovation is a global activity indicates that any new sector such as the BioEconomy will be global in its development. Hence countries should be reaching out to create international partnerships with others with a focus on similar aspects of the Bio Economy. The session will look at the connectivity of four countries with a focus on Biomass utilization from agriculture and forestry in their countries, but also recognize the need for a global connectivity to like-minded organizations. The Bio-based Delta in The Netherlands, Life Sciences Queensland, Australia, and Michigan Biotechnology Institute, United States of America have MOU's signed with Bioindustrial Innovation Canada. The MOU's have a focus on developing research and business partnerships for the development of biomass opportunities to bio-based chemicals; biomaterials; aromatics; lignin development and other areas of opportunity. The MOU's are in the early days of implementation of all the logistics, but are moving forward. The speakers will discuss their strengths as local groups and how the global outreach will add strength to their development of biomass to build their bio economy. 90 minutes Panel Willem Sederel
Mario Pennisi
Ludo Diels
Manfred Kircher
Murray McLaughlin - Moderator
1.4 Piloting a Course for Europe: The Support and Development Mechanisms Allowing the Bioeconomy to Take Off The drive for a greener, more sustainable economy is now a cornerstone for the continued development and prosperity of Europe. While many member states have published their own bioeconomy strategies, the European Commission views bioeconomy as an important response to facing key environmental and societal challenges. Research and development funding in the field has largely been solid but as with any new technology, one of the greatest issues occurs when this research comes out of the lab and into the “real world”. This session will hear from five key players in the support network established to specifically take companies through this difficult time and how these organizations are working together. The panelists will share their success stories from completed projects and insight as to how they see the bioeconomy growing and flourishing in the future. 90 minutes Panel Arno van de Kant
Kris Wadrop
Dirk Carrez
Brecht Vanlerberghe
Roger Kilburn - Moderator
1.5 Bioproducts Demands and Feedstock Security: Experiences for Inclusive Biorefinery Models The recent ICAO agreement pushes the demand for biojet fuels to new limits. A recent roadmap study in Campinas for example calculated the internal market need for Brazil to be 20 Mton in 2050. Multi-product biorefineries are seen as viable business options. However, existing 2G biorefineries show some major challenges in: 1) feedstocks availability & quality, 2) sustainability, and 3) value sharing & investments. Present business models for biorefineries heavily depend on low feedstock costs and reliable supply. Sustainable biomass production needs changes in agricultural practices that add value for biomass producers. Reconciling such targets within viable business models requires alignment of actors and inclusion of farmers and foresters in the value chain. In this session we will present and discuss experiences in the development of biorefineries in the US, Brazil, The Netherlands, South Africa and Jamaica. The main question of this session: How can biobased value chains become economically robust and secure long-term, sustainable supply of bioresources, improve agricultural management and align farmers’ values, interests, knowledge and concerns with the socio-economic and technical requirements of other partners in the chain? And at what scales? Experts will present opportunities, local specific challenges and solutions for the actual implementation of sustainable biomass value chains and discuss how to include biomass growers. We are keen to hear the audiences experiences and opinions and conclude with a session statement on this topic. 90 minutes Panel Oskar Meijerink
Hayo de Feijter
Hans van der Sluijs
Sjors Geraedts
Patricia Osseweijer - Moderator
1.6 Replicating Commercial Success in the Canadian Forest Industry This session will feature key opinion leaders as they discuss lessons learned from the successful commercialization of bioproducts in the Canadian Forest Industry. Panelists will share their experiences in scaling up novel technologies and how companies can replicate the success of other first movers while understanding and managing some of the barriers and risks inherent in implementing new and innovative technologies. 75 Minutes Panel Bruno Marcoccia
Balázs Tolnai
Richard Berry
Matthew Schacker - Moderator
2.1 Renewable Chemicals: Scale-up and Market Opportunities Tom Beardslee, Verdezyne VerdePalm Project Under Construction Verdezyne’s first commercial facility for the large-scale production of bio-based dodecanedioic acid, BIOLON DDDA, is under construction. Construction on the plant began in early 2017 at the Bio-Xcell bioindustrial park located in Nusajaya, Malaysia. Dodecanedioic acid (DDDA) is a linear, twelve carbon α,ω-dicarboxylic acid that has multiple applications in the chemical industry. Currently DDDA is produced using non-renewable fossil-based feedstocks such as butadiene or alkanes. Verdezyne has developed fermentation technology for the production of DDDA that uses renewable vegetable-based fatty acids. The largest application for DDDA is in the polyamide market to produce nylon 6,12 that is used for engineered plastics requiring special properties such as high chemical, moisture, and abrasion resistance. Other market applications include coatings, corrosion inhibitors, adhesives, lubricants, and fragrances. Ryan Smith, Origin Materials A Chemical Platform for the Production of Bio-PET The thermoplastic polymer, PET, is a product of the polymerization of monoethyleneglycol (MEG) and purified terephthalic acid (PTA). These monomers are sourced from fossil. To date, there are a few commercial efforts to produce bio-based MEG from ethanol, biobased PTA however, remains unavailable in the market. PTA is itself synthesized through the oxidation of isomerically pure para-xylene, whereas para-xylene is a product of oil refining. Origin Materials is a West Sacramento, California, chemical company, which converts biomass to commodity chemicals and resins. They have recently developed the technology, piloted the process, and will soon begin construction for a first-of-a-kind commercial demonstration plant that will convert biomass –not oil- to polymer grade p-xylene and other monomers of interest. This talk will introduce Origin Materials and aspects of its core technology. Cesar Granada, Earth Energy Renewables Improving the Value Proposition of Industries that Generate Organic Effluents and By-products with a Bolt-on Technology for the Manufacture of Carboxylic Acids A new technology that can bolt on to any of the industrial biotechnology manufacturing facilities is now available for converting the organics in the effluents and by-products into highly valuable carboxylic acids (i.e., fatty acids ranging from 2 to 8 carbons such as propionic, butyric and caprylic acids), which can substantially improve the economics of the present operations. This technology employs methane-inhibited anaerobic digestion (AD) followed by an efficient recovery process to produce these acids in a highly pure state. Unlike pure-culture fermentations, AD is an extremely robust and versatile process for bio-conversion of organic materials as it requires no aseptic conditions, no GMOs, and no extraneous enzymes. Philippa Davies, Tecnon OrbiChem Renewable Chemicals Market: A Changed Landscape This paper will present an overview on the commercialization and development of selected bio-based chemical building blocks in the past two years as companies refocus on their strategies amid low global petrochemical prices and changing regulatory landscape. The benefits, challenges and outlook for drop-ins and novel bio-based molecules will be discussed. 90 minutes Panel Philippa Davies
Ryan Smith
Cesar Granda
Thomas Beardslee
Jeff Passmore - Moderator
2.2 Biotech Protection in the US, Canada, and EU: Is This a New Era? 2017 may bring major changes to the patent systems of the US, Canada and Europe. In the US, the Trump administration has pledged to radically overhaul many areas of US policy, and the new USPTO Director will have the power to propose rules packages and issue internal examination guidelines. Will 2017 see the pendulum start to swing back in the direction of a more patent-friendly system in the US? In the EU, long awaited harmonized litigation in the form of the Unified Patent Court (UPC) is on the verge of going into force. Will the UK still be a part of the UPC despite Brexit, and, if so, how will Brexit affect the Unitary Patent as an EU right? The potential advantages and pitfalls of harmonized litigation in the EU will inevitably impact patent procurement strategies and considerations for invalidating and enforcing patents in the biotechnology industry. In Canada, the implementation of CETA has brought about reform to the Patent Act and the patent linkage regulations for pharmaceuticals. This panel will focus on changing views of our patent systems and how this will affect commercialization strategies for life sciences companies in the US, Canada and EU under a new era. 75 Minutes Panel Thomas Kurys
Hans Sauer
Kristel Schorr
Leonard Werner-Jones
Stephen Maebius - Moderator
2.3 Challenges and Dilemmas in Product Innovations and Funding Mechanisms in the Biobased Industry Jose Vitor Bomtempo, UFRJ Paulo Pavan, Fibria Which critical factors should be considered and how should they be analyzed in product innovations in the biobased industry? Apart from problems related to cost competitiveness versus fossil alternatives, innovators have challenges related to the identification, development and market adoption of new biobased products. Many questions must be considered concerning new products: Drop in or non-drop in? Final or intermediaries? Commodity or specialties? Platform chemicals? Biomass utilization efficiency? These dilemmas are not on-off decisions. The session will feature the Brazilian perspective, comparing visions from academia, research institutions and both established and new producers. Mark Riedy, Kilpatrick Townsend & Stockton LLP Creative US and International Debt and Equity Funding Mechanisms for Bioeconomy Technologies and Projects Available debt, equity and non-dilutive funding sources and mechanisms for bioeconomy technology companies will be presented, and their US and international projects, including the use of credit enhanced debt with government loan guarantees and other enhancements; green bonds; protective insurance products; strategic investor equity; non-dilutive grants and tax equity; regulatory incentives; capital and institutional markets; green funds from states and banks; and new capital equity expansion mechanisms such as MLPs, REITs, High Yield Bond Funds, Yieldcos, Warehouse Entities and other hybrid structures. This presentation particularly will examine program changes and new funding considerations in light of the new Trump Administration. Niels Schenk, BioBTX BioBTX: The Future of Aromatics BioBTX was founded to find an alternative, biobased and sustainable method for the production of BTX (benzene, toluene, xylenes and other small aromatics), high volume chemical building blocks normally made from petrochemicals. Since then, BioBTX has developed Integrated Cascading Catalytic Pyrolysis (ICCP), a proprietary process that allows processing of a wide variety of feedstocks and includes efficient output optimization. The key technologies allow biobased drop-in aromatics, higher aromatics, specialty products and deoxygenated bio-oil to be produced against market-competitive prices, in a proven and robust process from a variety of non-food feedstocks, including wood, glycerol, palm kernel shells, vegetable oils, agricultural residue, organic waste, etcetera. Integrated Cascading Catalytic Pyrolysis outperforms other processes for the production of aromatics because of 4 reasons. The first reason is that it is an integrated chemical process with very high efficiency that can easily be scaled up. Secondly all produced side products have value. Almost all carbon atoms are capitalized. The third reason is the wide range of biomass and waste streams that can be used. The fourth reason is that the main product is a direct drop-in, which allows fast growth because the downstream markets are fully deployed and growing. The past, present, and future funding is discussed in three different stages: feasibility, pilot plant, and commercial demonstration. Jeff Robert, FQPT How Many Start-Ups Before Commercialization is Realized Scale-Up and Innovation is not a singular event, but a series of events with unexpected results. As many of us are on the leading edge in our respective fields, we can only realize success by how we respond. In this presentation, FQPT will present how we have grown rapidly through unconventional Leadership, Partnership, and Client Service. 90 minutes Panel Paulo Pavan
Mark Riedy
Niels Schenk
Jeff Robert
Jose Vitor Bomtempo - Moderator
2.4 On track to Bio-based Aromatics from Lignin? The changing markets for pulp & paper, the advanced biofuels rise and the use of shale gas are only a few changes that prompt the biobased industry to look with other eyes to lignin and its use. It is no longer true that you can make everything out of lignin except money. Canada (FPInnovations and Bioindustrial Innovation Canada) and the BioInnovation for Growth platform (Netherlands, Flanders, NordRheinWestfalia) combined forces and experience between feedstock handling and chemical processing to make sustainable, safer and more performing BioBased Aromatics, paving the way to innovative polymers and materials. Representatives of these 4 countries will give there view and applications to stimulate discussions about the future. 90 minutes Panel Willem Sederel
Ludo Diels
Tom Browne
Manfred Kircher - Moderator
2.5 Waste Nutrients and Energy for Production of Microalgae and Other Industrial Microorganisms Many industries are looking for sustainable alternatives to fossil consumption and biomass has emerged as an attractive alternative. However, among all the challenges and issues of biomass being used to produce fuel and energy, securing the supply of biomass remains a critical factor for success and profitability. An industry producing its own biomass can overcome this problem. Many industries have CO2 stream, waste nutrients and waste energy that can be used to produce lipid-rich algae biomass or other microbial biomass for obtaining biofuel, bioenergy and renewable chemicals. This session will discuss two new case studies in Canada and present the results of a co-located algae production project conducted in Quebec with the dairy industry that looked to solve this industry challenge. 90 minutes Panel Stephen O’Leary
Julien Bley
John McGowen
Roberto Armenta
Simon Barnabé - Moderator
2.6 Industrial Synergies and the Circular Economy This session will examine how innovation and industrial synergies contribute to building a more circular economy. Specifically, the panelists will give an overview of what the circular economy means in the industrial sector; explain how urban waste and residues can be used to produce clean transportation fuels and renewable chemicals; and present concrete examples of how innovative synergies can be built with local municipalities and industrial partners to turn problems into solutions that help transition to a circular economy. The panel is composed of industrial leaders from the waste and chemical sectors, a circular economy think tank and a leading clean technology innovator. 75 Minutes Panel Laurent Spreutels
Peter Nieuwenhuizen
Ryan O'Gara
Timothy Cesarek
Marie-Helene Labrie - Moderator
3.1 Cluster Approaches to Biotechnology Canada is in a global innovation race. There is a need to create a comprehensive framework that aligns the country’s innovation agenda as well as the larger challenges like transitioning to a low carbon economy. The formation of a Biotechnology Innovation Cluster under this comprehensive framework will not only take advantage of the available sustainable biomass, it will build on the existing investment in micro-clusters and create an ecosystem that will foster the growth in completely new areas that build on Canada’s strengths. The session will hear from leading individuals and organizations driving the development of a national Industrial Biotechnology Innovation Cluster. Specifically, the panel will discuss how the Cluster plans to create entirely new industries while maintaining and strengthening existing strategic sectors such as forestry, agriculture and chemicals. 90 minutes Panel Perry Toms
Trevor Stuthridge
Marc Lepage
Jean-Pierre Martel - Moderator
3.2 Latest Developments In R&D Support for Forest Biorefinery Development In Canada This session will provide an overview of the uniquely Canadian approach in supporting forest biorefinery development by aiding the industry across the “Valley of Death” through new innovative technologies. These technologies include: a collaborative model, built on a coordinated approach by industry, academia, research centers and governments; the concept of maximizing the use of existing assets and infrastructure, in order to minimize the cost of initial transformative steps; large initiatives focused on a small number of well-defined objectives, based on the concept of innovation which implies concurrent R&D, engineering and market development, as well as close collaboration with downstream users and customers; and a national approach, supported by industry and the federal government, with regional-level implementation carried out in collaboration with the province. 75 Minutes Panel Minh Tan Ton-That
Eric Soucy
Fernando Preto
Michelle Ricard
Jean Hamel - Moderator
3.3 Protecting Innovations in Biofuels and Biobased Materials in the Face of Uncertain IP Policy The biofuels and biobased materials industries are growing rapidly. By some estimates, global biofuel capacity will grow to 61 billion GPY in 2018 and biobased products will make up 11% of the global chemical market by 2020. Yet these markets also face policy and legal risks, including an uncertain U.S. IP policy landscape and recent Supreme Court cases making it harder to protect biobased inventions. Panelists will discuss how uncertainty about the Trump administration’s IP and renewables policies and appointment of a new USPTO Director may affect innovation and IP enforcement. They will also address recent judicial trends that affect biobased inventions. Panelists will give insights on what these developments mean for companies seeking to safeguard biobased inventions, including what companies are doing to protect their technology from lab bench to scale-up. 90 minutes Panel Claire Schultz
Charles Collins-Chase
Christine Lhulier
Len Smith
Barbara Rudolph - Moderator
3.4 Scaling Up Renewable Natural Gas: How to Move Forward? Scaling up renewable fuels has proven challenging. Biogas offers both clear advantages and disadvantages, as a renewable fuel and bioenergy carrier. This panel will present the state of the commercial practice in producing, aggregating, transporting and utilizing renewable biogas/biomethane. Further, the session will focus on how to overcome current challenges to biogas scale up so that this versatile fuel can more fully achieve its potential. 90 minutes Panel Joachim Pheiffer
Fabrizio Sibilla
Johannes Escudero
Brian Foody
Harrison Clay
Rebecca Boudreaux
Bruce Dale - Moderator
3.5 Achieving Radical GHG Emissions Reductions from Novel Biorefinery Value Chains Innovation is at the heart of the emerging bioeconomy - new feedstocks, new technologies, new markets and new business models. The bioeconomy represents opportunity and risk. In Canada and around the world, novel value chains are being conceived. How can these new value chains help contribute to radical reductions in GHG emissions? How do funding agencies assess GHG reduction potential and what are the challenges technology developers face addressing this evaluation? By gathering different perspectives from leading industry associations, funding agencies and technology developers, this session will seek to explore linkages between biorefinery value chains and potential for GHG emissions reduction. 90 minutes Panel Robert Larocque
Ziyad Rahme
Pierluigi Picciotti
Virginie Chambost
Frederic Clerc - Moderator
3.6 Advanced Bioethanol Technologies Bioethanol production is well established globally; from first generation corn and sugarcane ethanol to the continuing progress with the commercialization of second generation ethanol from biomass. The emergence of new technologies for production and utilization of bioethanol will increase the range of feedstocks available for production and open up new markets that overcome blend wall constraints. This session will explore the opportunities for new ethanol technologies and markets driven by the emergence of advanced bioethanol technologies. 75 Minutes Panel Mikio Matsumoto
Martin Mitchell
Alex Baker
Ian O'Hara - Moderator
4.1 Microalgae: The Solution for Sustainable Ingredients in Animal Feeds Microalgae provide a solution to sustainably improve the quality of the animal feeds produced. Currently, animal feed ingredients from microalgae have been approved and are on the market for use in feed formulations. As populations increase and resources become limited, microalgae are well positioned to fill gaps in production of essential nutrients. With short production cycles and the ability to grow to very high densities in production systems, microalgae offer a sustainable solution to an expanding problem of how our food and feeds will be sustainably produced in the future. This session will hear from a select group of microalgae feed ingredient producers as they showcase the diversity ingredients that can be produced by microalgae to enhance animal feeds. 90 minutes Panel Len Smith
Amha Belay
Geoff Horst
Xun Wang
Valerie Harmon - Moderator
4.2 Canadian Biomass Supply Chain Improvements to Increase Investment Opportunities With a focus on hemp, flax and purpose grown crops, a network of Canadian crop producers, research organizations and industry has emerged to identify and overcome critical challenges that prevent commercial investment in the processing of Canadian biomass for industrial bioproduct applications. This session will expand upon the near term opportunities that have been identified, the plans that have been prepared and the work that has commenced in the following areas: enhancing fiber quality through controlled biomass retting practices; removing man-made materials and improving the compaction of straw bales; improving the efficiency of harvesting and baling operations; developing viable needs rational for purpose grown crops; and reducing risk and improving investment decision-making through use of reliable geographic maps containing biomass quantity and quality information tied to economic and industrial data. 75 Minutes Panel Jan Slaski
Hank Froese
Jordan Solomon
Roger Samson
Sean McKay - Moderator
4.3 Carinata: Expanding the Oilseeds Supply Chain Agrisoma, developer of commercial varieties of Brassica carinata as a renewable fuels feedstock and sustainable protein source, has aligned with a diverse and committed group of supply chain partners.  From agricultural producers to feedstock and fuel processors and logistics providers, the supply chain leverages existing businesses with opportunities to expand business, markets or margins at each step.  A commitment to sustainability and focus on reduction of GHG emissions, beginning at the farm, is paramount to the value proposition and accrues across the supply chain.  Innovative approaches to cropping systems mean more efficient use of existing farmland and additional revenue opportunities for farmers.  A certified sustainably produced and non-GMO oilseed with high oil content and high protein represents the potential for differentiated feed markets and improved processing economics.  An agriculturally produced industrial oil with GHG reduction that rivals that of waste streams represents an expanding source of feedstock to meet capacity expansions for biojet and renewable diesel and accelerating regulations requiring volume and emissions reductions.  Developing a supply chain that recognizes the potential of Carinata has created an opportunity for meaningful expansion in the coming years.  The Panel includes perspectives on Carinata from those who have been involved in the regulatory and sustainability aspects, to the those who have used Carinata in their commercial biofuel supply chain. 90 minutes Panel Martin Vidal
Rolf Hogan
Christophe Beaunoir
Steve Fabijanski
Jim Lane - Moderator
4.4 The Unified Field Studies of the Algae Testbed Public Private Partnership: What Did We Learn and Where Are We Going? From 2013 to 2015, the Algae Testbed Public Private Partnership conducted a series of coordinated field trials termed the Unified Field Studies (UFS) in five distinct geographical locations across the US. This effort was the first of its kind and has provided the industry with a robust public data set to identify solutions to reduce the barriers for production of biomass for biofuels and bioproducts from microalgae in open ponds. This session will examine the challenges that were overcome in coordinating this effort at various locations to produce quality data. Additionally, the panel will present UFS data of biomass productivity, biomass composition and production system reliability. The examination of this data will provide a comprehensive look at what was learned and how to utilize this research to advance the future of microalgae as a feedstock for biobased industries. 90 minutes Panel Lieve Laurens
Valerie Harmon
Edward Wolfrum
John McGowen - Moderator
4.5 Building the Value Chain for Biomass: From the Farm to the Consumer and the Need for Partners In agriculture, farmers, being an entrepreneurial lot, are constantly looking at how they can create more value at the farm gate. In the past this has been done with improved varieties, agronomic practices and marketing, where as in the future the efficiencies will be based on improved management, innovation and new market opportunities, such as biomass. When we look at the future of agriculture, it is about maximizing use of our crops (both food and nonfood); it is keeping our environment healthy; it is about managing the carbon molecule to reduce green-house gas emissions by building a bio-based products sector. Based on this a group of farmers in Ontario formed the Cellulosic Sugar Producers Coop (CSPC) and contracted Bioindustrial Innovation Canada (BIC) to assess technologies and assist with selection of a company to build a Sugar mill in Sarnia. The company selected by CSPC was Comet Biorefining. Comet has partnered with the coop and offtake partners, BioAmber, Inc. to allow them to move the commercial development forward. The objective of the CSPC is to see a complete value chain for sugar from corn stover to consumer products in Ontario in the future. The Value chain started with BIC as the cluster developer and enabler; which then linked with farmers to form a Coop and study to assess technologies. From this Comet was selected as the corn stover to sugar producer and they did offtakes with BioAmber, Inc. to use the sugar. Mitsui & Co is a trading company and partnered with BioAmber, Inc. in a JV called BioAmber Sarnia, Inc. Mitsui and Comet are working together to market biomass sugar in the bio chemical industry. The story of establishing this value chain will be reviewed in this session. 90 minutes Panel David Park
Ken Beach
Mike Hartmann
Andrew Richard
Sandy Marshall - Moderator
4.6 Biomass Quality Network Canada: Agricultural Biomass Quality Standards for the Biochemicals, Bioenergy, Biofuels and Biomaterials Sectors Leftover agricultural biomass provides innovative opportunities for developing sustainable green technologies. With the rising interest in using residual biomass as feedstocks for industrial sectors such as biochemicals, bioenergy, biofuels and biomaterials, there is a need to better understand the quality standards the biomass must meet for commercial implementation. The Biomass Quality Network Canada (BQNC) was assembled to facilitate the development of biomass quality standards and test methods in the industrial sectors of biochemical, bioenergy, biofuels and biomaterials. This session will hear from industry sector leaders as they provide a brief overview of the existing gaps in quality standards and the activities underway to address developing industry methods and standards. 75 Minutes Panel Ray Bergstra
Alvin Ulrich
Babak Owlam
Stu Porter
Lin-P'ing Choo-Smith - Moderator
5.1 Utilizing Synthetic Biology to Address Global Challenges Bruce Dannenberg, Phytonix Vikramaditya Yadav, The University of British Columbia Peter Lindblad, Uppsala University Utilizing Synthetic Biology to Address Global Change This panel will discuss how companies are employing synthetic biology to address global challenges such as climate change and the production of cost competitive, sustainable chemicals and fuels. Also to be discussed are the economic and environmental opportunities that new advances in industrial biotechnology, enabled by synthetic biology, provide in terms of a rapid transition to a new bio-economy and ultimately to a CO2-based economy for the production of consumer products, chemicals and fuels. Andy Bass, Intrexon Corporation Synthetic Biology to Influence Change Humankind is inventing modern methods for programming biology to sustainably manufacture many of the products we use and consume in today’s society. Bio-based production leverages biological efficiency at an industrial scale to create new, high performance, bio-derived products, which are healthy for the planet and humankind. In order for these products to become widely used, consumers must understand and believe in the benefits. As a result, the roles of creative designers and educators are essential to influence people to adopt these new products for the betterment of Earth, for our generation, and those to come. 90 minutes Panel Nick Bourdakos
Peter Lindblad
Vikramaditya Yadav
Andy Bass
Bruce Dannenberg - Moderator
5.2 Synthetic Biology: The Emergence of a New Value-Chain? The last year has seen unprecedented public and private investment in a whole new set of synthetic biology companies. Concurrently, 2016 has seen intense deal-making within the synthetic biology ecosystem, specifically between synthetic biology companies and more traditional players in the chemicals industry. The business models of these new entrants in the industrial biotech sector can be viewed as different from the first wave of companies. This session will feature synthetic biology companies with a focus on DNA synthesis, enzyme and protein engineering, strain construction and optimization and gene editing as they seek to answer such questions as: Are we witnessing the emergence of a new value-chain and a new "division of labor" to bring renewable chemicals on the market? How will the ecosystem of industrial chemicals be impacted? 75 Minutes Panel Vonnie Estes
Alexandre Zanghellini
Jason Kelly
Emily Leproust
James Iademarco - Moderator
5.3 Microbial and Synthetic Approaches to H2 Metabolism for CO2 Utilization Blaine Metting, Science Advisor-Green Earth Institute Inc., Tokyo; Global Advisory Board Member, CO2 Sciences, Inc., USA Overview of autotrophic and heterotrophic microbial systems, synthetic biology and in vitro options for CO2 uptake and industrial utilization focusing on potential for global climate relevance by different upscaling approaches. Hideaki Yukawa, Utilization of Carbon Dioxide Institute Inc., Tokyo, Japan Production of Biofuels and Bio-chemicals from CO2 using Hydrogen Bacteria Utilization of Carbon Dioxide Institute Inc (UCDI) develops innovative biotechnologies based on hydrogen-utilizing bacteria to convert CO2 into high-value products to address food security and global warming. An initial focus on food security is to develop and manufacture microbial animal and aquaculture feeds (biofeeds). This novel, patented technology is also applicable to producing biofuels (ethanol and butanol) and bio-chemicals (organic acids and amino acids). Carolina Zampol Lazaro, Department of Microbiology, Infectious Diseases and Immunology, University of Montreal Overview of Microbial Hydrogen Production Utilization of CO2 requires initial reduction with the necessary reducing power provided in a variety of ways. One attractive and versatile possibility is the use of hydrogen, produced in a sustainable and renewable way. The promises and limitations of various hydrogen generating microbial processes that could accomplish this are discussed, including direct generation from water and the potential for microbial extraction of hydrogen from different waste streams. Presently, technical barriers limit practical application in this area. Specific examples of strategies to increase conversion yields through co-cultures using microbes with complementary metabolisms, or novel fermentation strategies, will be given. Alex Beliaev, Pacific Northwest National Laboratory, Richland WA Ultrafast Growing Cyanobacteria for Fuel and Chemical Production Efficient coupling of photosynthesis and productivity is central to solar-based biotechnology. Understanding factors constraining carbon processing rates is necessary to overcome productivity constraints. Using ultra-fast growing cyanobacteria, research conducts systems-level modular analysis of photosynthetic energy conservation and reductant generation, CO2 fixation, photosynthate production and biosynthesis of metabolic intermediates. Coupled with regulatory network reconstruction, this approach provides testable predictions of reductant fluxes, reductant partitioning to carbon metabolism and other sinks, and biosynthetic pathways for synthesis of fuels and chemicals. This presentation highlights how understanding unicellular cyanobacteria that achieve ultrafast growth rates through physiological acclimation and effective management of metabolic resources opens prospects for industrial technology development. 90 minutes Panel Hideaki Yukawa
Carolina Zampol Lazaro
Alex Beliaev
Ed de Jong
Blaine Metting - Moderator
5.4 An Ecosystem to Rapidly Deliver Commercial Bioprocesses Full Session Abstract PDF Link 90 minutes Panel Colin South
Claes Gustafsson
Abhay Singh
Kevin Craig
Martha Marrapese - Moderator
5.5 Expanding Synbio Platforms for High Value Products Moderator- Daniela Quaglia, PLOS Synbio Derek Greenfield, Industrial Microbes Expanding Synbio Platforms for High Value Products To date synthetic biology has relied on commonly used organisms, such as E coli and yeast for the production of mammalian, plant or bacterial genes in order to make commercial products. These platforms are not always the best suited for complex products or industrial chemicals. The session will look at examples of companies and academics that are looking beyond the traditional to new platform organisms and technologies and new types of products to bring down cost of production, improve robustness and to introduce new products and materials to the market. Lori Giver, Calysta Producing Protein and Chemicals from Methane, a Sustainable Platform for Biotechnology Calysta, Inc. has developed a robust bioconversion platform utilizing host organisms (methanotrophs) capable of metabolizing methane to a variety of products including higher value biochemicals and single cell protein. Calysta’s first commercial product is FeedKind® protein, a sustainable, traceable alternative feed ingredient for fish, livestock and pet nutritional products. FeedKind protein is comparable in many respects to high-quality fishmeal, a critical ingredient for aquaculture. FeedKind protein is sustainably produced via non-GMO fermentation of methane, which has minimal impacts on water and land use and does not compete with any aspect of the human food chain. Guy Helin, Syngulon Synthetic Biology Technology to Boost Green Chemicals Production Syngulon is developing a patent portfolio of genetic technologies focusing on the control of industrial microrefineries to solve key issues such as genetic security, yield increase, genetic drift, contamination prevention and genetic safety. The first patent of Syngulon, US Patent 9,333,227 issued in May 2016, covers the use of bacteriocin/immunity in any bacteria, yeast or algae for the controlled growth of microorganisms. Syngulon is presenting the latest developments of their synthetic biology technology to boost Green Chemicals production. William Bardosh, TerraVerdae Bioworks Engineered Performance Biomaterials and High Value Bioproducts TerraVerdae Bioworks is developing an integrated bioprocess for the production of PHA biomaterials and high value bioproducts from single carbon (C1) feedstocks. Utilizing an integrated approach for bioprocessing, systems biology and biopolymer development, we have demonstrated the commercial scalability of our bioprocess and are developing value added finished products for personal care, agriculture and specialty biomaterials markets. 90 minutes Panel William Bardosh
Guy Helin
Derek Greenfield
Lori Giver
Daniela Quaglia - Moderator
5.6 Synthetic Biology Used to Design and Manufacture Renewable Chemicals Ian Fotheringham, Ingenza Ltd., Roslin, United Kingdom Deployment of Integrated Synbio Tools to Enable Biopharmaceutical Production Systems In my presentation I will describe the combination and application of Ingenza’s proprietary suite of inABLE® synthetic biology tools to increase the speed and predictability with which mammalian cell lines and microbes can be engineered as efficient production systems for important protein biopharmaceuticals. I will describe specific commercially relevant examples to show how these methods address critical needs in providing lower cost manufacturing to meet vastly undertreated patient populations (Haemophilia/Factor VIII) and the discovery and production of novel bacteriocin antibiotics (Epidermicins) to address multi-drug resistant bacteria. Andrey Zarur, Greenlight Biosciences Novel Synthesis of Double-Stranded RNAs Double-stranded RNAs (dsRNA) are the key molecules used in the majority of RNA-based interventions, including RNA interference for agricultural, veterinary and human health applications, as well as messenger RNA products used in trait development or human therapeutics. Synthesis of dsRNA is a major obstacle hindering the development of RNA-based products for broad applications due to extremely high synthesis costs, low yields and difficult downstream separations. Several novel bio-based technologies promise to make dsRNAs available for a variety of low cost applications, ranging from crop protection to animal vaccines, from trait introduction into plants to human healthcare. Dara Djafarian, Creatus Biosciences Inc. Enabling Xylose Potential - A Biobased Platform for Increasing Product Yield from 2nd Generation Feedstocks Over the past decade, the technology to breakdown lignocellulosic biomass to constituent sugars has improved dramatically, making second generation production of valuable compounds viable, though it remains an economic challenge. Most bio-based platforms can assimilate glucose effectively, however, the same platforms often falter in processing the second-most abundant sugar in lignocellulosic biomass, xylose, which accounts for 10%-30% of total sugars. Enabling xylose potential is one strategy developed on Creatus’ bio-based platform. Improving xylose uptake by the cell and boosting the activity of xylose metabolizing enzymes increases yield in a product-agnostic manner and improves economics. Kari Koivuranta, VTT Technical Research Centre of Finland Ltd. Assessing the Fast Development of Production Platforms for Renewable Chemicals Genetic engineering of novel species for production of desired products may be challenging. To speed up development of production platforms VTT has developed CrispR technology for a broad range of eukaryotic microbes. Additionally, VTT has developed universal synthetic expression system (SES) which allows for high level, constitutive expression in diverse growth conditions and growth stages. This system is based upon a synthetic transcription factor (sTF) that regulates expression of target gene via a sTF-dependent promoter. Rob Brown, Synthetic Genomics Harnessing the Power of Nature to Address Global Challenges Today, many products we know, use and consume are produced by biological systems — from medicines to nutritional products to chemicals. Understanding nature’s design rules, how living organisms have evolved, and how they function allows smooth transition from in silico design to DNA synthesis and assembly. Here I show several examples where we have developed novel production systems by engineering and optimizing both the cell specific productivity as well as the manufacturing process. We continue to leverage to the power of mother nature to revolutionize the current paradigm in bio-based production. 90 minutes Panel Rob Brown
Andrey Zarur
Dara Djafarian
Kari Koivuranta
Ian Fotheringham - Moderator
6.1 Performance Enzymes for Food Ingredients Vince Sewalt (Panel Moderator), DuPont Industrial Biosciences Development and Commercialization of Performance Enzymes Commercialization of industrial enzymes that are cost-effective and tailored to the customer application requires a suite of integrated capabilities to develop and operate microbial cell factories producing protein -engineered enzymes. Getting such products in the hands of customers in a timely manner also requires capabilities to streamline safety assessment and approval in a complicated global regulatory environment. DuPont Industrial Biosciences excels in these areas, as exemplified by our G4 amylase technology development and market access success. Additional industry examples will be reviewed by other panel members who will discuss techniques such as protein engineering, synthetic biology tools for enzymes used in various applications. Marc Struhalla, c-LEcta GmbH Cell-free Synthetic Biology as Key Enabling Technology for Novel Food Ingredients Many technology companies in the industrial biotechnology arena are nowadays focusing their activities on building microbial production strains to address new ingredient products for the food and personal care industry. Production hosts made from bacteria or yeast are designed and engineered to be applied in fermentation processes to manufacture novel sweeteners, flavors or fragrances. Enzymes can be easily adapted to cope with very high substrate and product concentrations that are by factors larger than usual titers encountered in fermentation processes. The presentation will exemplify enzyme engineering and synthesis process development work done by c-LEcta on multi-enzyme one-pot synthesis of high-value food ingredient products as well as an outlook towards the potential of cell-free synthetic biology will be given. C. L. Rathi, Advanced Enzymes Technologies Ltd Enzymatic Extraction of Crude Palm Oil from Palm Fruits Palm oil is one of the largest processed and consumed oil in the world. It is currently extracted using thermo-mechanical process, which has disadvantage of sub-optimal oil yield. To overcome yield loss challenge the enzymatic palm oil extraction process was developed. Enzyme formulation was designed and optimized to degrade cell walls of the palm fruits resulting in increased Oil Extraction Rate (OER). Andrew Ellis, Biocatalyst The Latest Tools and Technologies for Enzyme Discovery and Development Advancement in novel enzyme development has been underpinned by step changes in the areas of genetic engineering, protein engineering, rapid enzyme discovery and strain developments. The presentation will focus on some specific state of the art tools and technologies and also highlight some bottlenecks for further innovation. It will conclude with a glimpse into the future of biotechnology. John Perkins, DSM Bright Science at DSM: Use of Enzymes in Food Application Enzyme production and engineering is one of the key technologies needed to improve the quantity, quality, and enjoyment of food. DSM utilizes a number of microorganisms to produce food-grade enzymes that can be applied to baking, dairy, oil and fruit processing applications. Moreover, engineering of enzymes is used to introduce new and improve functionality, allow cost-effective improvement of food-base processes. These genetic engineering tools include site-directed mutagenesis based on rational targeting of functional amino acids coupled with high-throughput screening technologies and more recently genomic and bioinformatics tools that provide a wealth of structural and sequence data that allows for smart protein engineering. An example from DSM’s enzyme food application will be presented. 90 minutes Panel Chandrakant Rathi
Marc Struhalla
Andrew Ellis
John Perkins
Vincent Sewalt - Moderator
6.2 Synthesizing Specialty Ingredients for Cosmetics, Flavors, Food Ingredients, Fragrances Full Session Abstract PDF Link 75 Minutes Panel Benoit Palms
Emmanuel Petiot
Pramod Kumbhar
Rainer Figge
David Demirjian - Moderator
6.3 Engineering Flavors and Fragrances Molecules Moderator- Gwen Rosenberg, Rosenberg Business Communications Toine Janssen, Isobionics Engineering and Producing Flavor and Fragrances Based on Fermentation The presentation will focus on the steps from engineering to producing flavor and fragrances based on fermentation. Some of the natural ingredients used today are scares or compromised by diseases. The biotechnology platform gives a solution to those problems. Engineering, developing and producing Natural flavor and fragrances requires many careful steps to commercialize organoleptic successful products for the flavor and fragrance market. Edi D. Eliezer, Conagen, Inc. Fast track Technology Transfer from R&D to Commercial Production of Renewable Food and Flavor Ingredients: Global platform & RebM. Current biotechnologies allow a paradigm shift in sustainable and cost-effective production of natural ingredients by synthetic biology, advanced fermentation and bioconversion technologies. The key to a fast track success is company’s vertical integration from discovery to commercialization. We leveraged our strategies in one of the most lucrative F&F industry segments: the $90 billion global sweetener market. Our recent success story is the fast-track development, FDA approval and cost-competitive manufacturing of renewable Rebaudioside M (RebM), the most desirable molecule of Stevia based natural sweeteners. The RebM has very low/ zero calories, greater sweetness, no bitterness and superior sensory profile compared to other steviosides. Alexander Oelke, Lonza Ltd Large Scale Microbial Fermentation Services for the “White Biotechnology Industry” Backed by Lonza’s more than 30 years’ experience and expertise in biological processing and its track record in the chemical and biotechnological industry, Lonza offers its customers a full “one-stop-shop” service package at all stages of development and manufacturing of their microbial fermentation products. Out of its labs in Visp, Switzerland, Lonza offers all services in the context of initial strain optimization and process development. Processes ready for production, either developed in Visp or provided by Lonza’s customers, are transferred to the manufacturing site in Kourim, Czech Republic, for final scale-up and pilot runs. Once commercial production has started, thorough, continuous process optimization is a key-element for Lonza in order to support the customer in staying competitive and to maximize his market success. 90 minutes Panel Toine Janssen
Alexander Oelke
Edi Eliezer
Gwen Rosenberg - Moderator
6.4 Opportunities to Produce Nutritional Value Products Full Session Abstract PDF Link 90 minutes Panel Steve Hartig
Jacques Beaudry-Losique
Sean O'Connor
David Anton
Scott Chaplin - Moderator
6.5 Harnessing Industrial Biotechnology to Meet Aquaculture Feed Needs Much of the world fish stocks are in rapid decline and a major source of the decline in stocks is demand for fish meal and oil that typically ends up in feed for aquaculture. At the same time, demand for fish for human consumption continues to grow rapidly as a healthy alternative to beef, swine and poultry. This session will highlight the need for renewable and biobased nutrients as well as feed additives to support the growth of the Global Aquaculture Industry. Panelists will review and discuss perspectives from start-ups, mature companies and development groups regarding the future feed and nutrient needs of aquaculture as well as approaches underway to meet these needs by harnessing industrial biotechnology. 90 minutes Panel Josh Silverman
Andre Dumas
Larry Feinberg
Bryan Tracy
Joel Stone - Moderator
6.6 Microalgae Ingredients for Foods: A Biomass Source to Enrich the Biobased Foods Industry Microalgae provide a unique and diverse set of organisms for use as food or in foods as food ingredients and additives. Our distinguished panel will bring some of the worlds first algae producers to enter food markets together in order to elucidate the potential of this new and exciting resource. Microalgae are not only diverse, but are a sustainable alternative for many of the ingredients currently sourced from non-renewable resources. Various pigments and essential nutrients are manufactured by primary producers or plants, and microalgae offer the advantage of short production cycles (7 days as opposed to one crop annually) to obtain these vital nutrients for our foods. The microalgae industry is currently poised to become a major supplier of biobased, sustainable foods and food ingredients. 75 Minutes Panel James Irwin
Xun Wang
Ross Zirkle
Rebecca White
Matt Carr - Moderator
7.1 Sustainable Pathways to Furanics, FDCA, and Design of New Polymers Moderator-Jim Barber, Barber Advisors LLC Stephan Roest, Corbion Corbion’s Proprietary Route to 2,5-Furandicarboxylic Acid (FDCA) from Renewable Resources Corbion has developed a proprietary process to produce 2,5-Furandicarboxylic acid (FDCA) from renewable resources. Corbion uses a biocatalytic process to convert FDCA from HMF, obtaining high conversion yields and selectivity. FDCA has the potential to replace purified terephthalic acid (PTA) in a variety of applications. For example, combined with monoethylene glycol (MEG), FDCA can be polymerized into polyethylene furanoate (PEF). PEF gives improved finished product performance, due to better barrier, thermal and mechanical properties when compared to PET. Jesper van Berkel, Synvina Packaging Opportunities for PEF Synvina, a Joint Venture of Avantium and BASF, produces and markets FDCA and PEF. Synvina aims to become market leader for FDCA and PEF and to realize this ambition intends to construct a plant at the BASF Verbund site in Antwerp, Belgium, with a capacity of up to 50,000 metric tons of FDCA per year. PEF is a 100% bio-based and recyclable polymer which can be used for a wide variety of packaging applications. Synvina’s pilot plant in Geleen, The Netherlands, has been producing FDCA using the YXY® process developed by Avantium since 2011, giving Synvina and its partners ample know-how and experience in effective high quality PEF production as well as its various end-use applications, such as bottles, films and fibers. Michael A. Saltzberg, DuPont FDME and PTF: Furanic Materials for Improved Barrier Packaging Performance DuPont and ADM have jointly developed a breakthrough process to produce furan dicarboxylic methyl ether (FDME) from fructose utilizing a chemical process that integrates the dehydration and oxidation steps. This integration provides higher yields, improved energy utilization, and requires fewer unit operations, thus substantially reducing the required capital investment. A pilot plant to demonstrate and refine the process at large scale is currently under construction at ADM’s Decatur, IL facility and will start up later this year. It is planned to integrate the commercial FDME facility with one of ADM’s world-scale corn processing facilities to provide low cost, high purity fructose based on ADM’s years of expertise in this area. This talk will give an update on these two efforts to develop a new renewable monomer and polymer for barrier packaging applications. 90 minutes Panel Michael Saltzberg
Jesper Van Berkel
Stephan Roest
Jim Barber - Moderator
7.2 Renewable Chemicals and Thermoplastics for Performance Materials Marcel Lubben, Reverdia Natalie Bittner, Covestro Stefano Facco, Novamont SpA Mateus Lopes, Braskem Renewable and biobased chemicals have the potential to reduce greenhouse gas emissions and create opportunities for materials across markets. The session will discuss news and developments from leading renewable chemicals companies. In the electronics and footwear market, biobased thermoplastic polyurethane (TPU) offers excellent physical properties while also providing a superior carbon footprint. Bio-TPU can be produced using bio-succinic acid as a raw material. As part of a unique value chain model, Reverdia and Covestro have taken application co-development and promotion of TPUs to a new level. Novamont’s presentation will focus on Mater Bi, a family of innovative biodegradable and compostable bioplastics, as well as on recent newly started industrial production sites, offering a variety of renewable chemical building blocks, like 1.4 BDO, C9 mono and dicarboxylic acids (Pelargonic and Azelaic acid). Braskem’s Green Butadiene development project in cooperation with Genomatica and the new biotechnology projects of Braskem will be highlighted during the session. Chris Ryan, Gevo, Inc. From Ecosystems to Large Scale Commercial Processes for Renewable Jet, Isooctane, & PET Large scale manufacturing of renewable isobutylene, jet fuel, p-xylene, and poly(lactic acid) have all been successfully developed 'on purpose' beginning with a clear definition of the business target and a technology approach which combines cutting edge biotechnology with efficient chemical processes. The business target and technology approach form the basis of a roadmap that sets the direction and milestones around which an ‘ecosystem’ of development collaborations can be created to create commercial value. The key to success of the ecosystem is held by the company who is driving the development and operation of the manufacturing process and also interfaces with the market. This talk will highlight examples of the importance of cross-company collaborations to access the best biotechnology tools to address challenges encountered in large scale bioprocesses. 75 Minutes Panel Natalie Bittner
Mateus Lopes
Stefano Facco
Christopher Ryan
Marcel Lubben - Moderator
7.3 Scaling Novel and Innovative Processes for Commercialization Scaling novel and innovative processes to produce renewable chemicals, bioplastics and biobased materials is a major and important step towards the commercialization of these products. However, doing so requires the combination of many steps and many participants to work closely together to move successfully over what is frequently referred to as “the Valley of Death”. This session will provide insight towards this effort from the perspective of the key stakeholders in this process: an Engineering/Procurement/Construction (EPC) firm; a Technology Developer; a Current Producer; and a Contract Research Organization (CRO). 90 minutes Panel Todd Pray
Cecil Massie
Robert Kress
Nick Bourdakos
Bryan Yeh - Moderator
7.4 Advancements in PLA and PHA: Synthesis and Improved Performance Full Session Abstract PDF Link 90 minutes Panel Rich Weber
Derek Atkinson
Mark Herrema
Puneet Trehan
Philip Goodier
Marc Lankveld - Moderator
7.5 Generating Customer Value with Renewable Chemicals John Shaw, Itaconix Corporation Peter Nieuwenhuizen, AkzoNobel Greg Smith, Croda Chris Guske, D-Squared Biotech Consulting Robert Nolles, Cosun Biobased Products The Unbeatable Beet: Creation of an Integrated and Cost-Effective Cascading Biorefinery To make better use of sugar beet pulp, Royal Cosun invested in an integrated and cost-effective cascading biorefinery to refine sugar beet pulp and isolate high-value components for use in a broad variety of end products. The overall objective of the biorefinery initiative is to establish value chains based on microcellulose fibers, arabinose, and galacturonic acid in high-value markets such as detergents, paints, coatings, and composites—as well as in personal care and the oil and gas industries. Cumulatively, the three main constituents of sugar beet pulp account for 65% of its mass, and the residuals of the biorefinery are suitable to generate biogas. 90 minutes Panel Christopher Guske
Peter Nieuwenhuizen
Greg Smith
Robert Nolles
John Shaw - Moderator
7.6 Meeting Brand Owner and Retailer Demand for Green Chemicals, Materials and Products Through Renewable Chemicals and Biobased Materials Many brand owners and retailers face regulatory, consumer and NGO pressure to eliminate certain chemicals or materials of concern from the products they make or sell, and at the same time, they are under competitive pressure to identify new chemistries and materials to achieve new or improved product performance without introducing new chemical hazards. Renewable chemicals and biobased materials offer brand owners and retailers promising new solutions to both of these business challenges. This session will feature two brand owners as they discuss the challenges faced in finding new, safe and effective chemicals and materials for their products; a supplier that will provide their perspective on targeting their development and commercialization efforts to the needs of brand owners and a business organization’s collaborative efforts to support green, renewable and biobased chemistry innovation in startups and established companies. 75 Minutes Panel Rusty Pittman
Karen Graziano
Timothy Staub
Derek McPhee
Joel Stone - Moderator
Activation of Neutral Oligosaccharide Feedstocks by Dual-Enzymatic Oxidation There is a growing need to find alternatives to petroleum-based chemicals and materials. Lignocellulosic biomass could help to address this issue as it is composed of modifiable carbohydrate polymers, including hemicellulose (~30%), which is accessible through waste streams in wood processing industries. The approach being taken to make hemicellulose-based polymers is to replace specific sugar hydroxyl groups with more reactive nucleophiles in order to “activate” these sugars. This activation will allow for subsequent modifications yielding unique biobased polymers. Enzymatic oxidation has been shown to be a viable route to regioselectively introduce more reactive functionalities onto sugars with advantages over chemical approaches such as no loss of molecular weight, no protective groups, and mild reaction conditions. Therefore, the final goal of this work will be to develop a simultaneous two-enzyme treatment to efficiently introduce oxidized sites onto waste hemicellulosic sugars to allow for upgrading to high value biobased materials. This approach will have environmental process advantages as well as likely improved material biodegradability and biocompatibility due to preservation of the unique sugar structure in the polymer backbone. 90 minutes Poster Spencer Imbrogno
A New Approach to Evaluate Enzymes for Biotechnology in Regard to pH and Temperature Glycoside hydrolases are important for various industrial applications. The pH- and temperature optima of these enzymes or enzyme mixtures are commonly determined in two separate measurements. This results in a two-dimensional assessment of the enzymes optimal pH value at only one temperature and vice versa. However, this does not allow to predict an enzymes characteristic at any desired pH/temperature combination, if this activity was not directly determined by at least one of the two measurements. We developed a one-step method to determine both optima simultaneously by measuring 96 different combinations of pH and temperature in parallel. Subsequently, the obtained data are converted into a three-dimensional contour plot, which displays the pH value and temperature of the multiple reactions in relation to the activity of the enzyme. Using this three dimensional graph, the activity of the enzyme at any combination of pH value and temperature within the broad test conditions can be readily read out. Single enzymes as well as enzyme mixtures can be used. Different assays with various substrates have been validated. The assessment whether an enzyme or enzyme mixture is suitable for any combination of temperature and pH is possible at a glance. The proposed method offers several significant advantages over existing methods and visualizes the complete correlation between temperature, pH, and activity of an enzyme. 90 minutes Poster Jonathan Herlet
An Extraction Method and a Formula of Non-allergic Polysaccharides from Fungi with Immune Modulatory Property Fungi provides bioactive compounds in which several polysaccharides are claimed to possess immune modulatory and anti-cancer properties. In this study, a method to effectively extract polysaccharides from mycelia was demonstrated, as well as a formula of polysaccharides from a variety of fungi species was established for the uses of boosting immune system. The polysaccharides produced from this process has successfully removed protein, peptides and other macromolecules which may trigger an allergic reaction. The formula of the polysaccharides was a combination of extracts from Ganoderma lucidum, Coriolus versicolor, Lentinula edodes, Hericium erinaceus, Pleurotus eryngiig and Flammulina velutipes. 90 minutes Poster Chanchan Wang
A Revolution in Biobased Products and Packaging Global demand for renewable materials has led to a shift in the production of sustainable, biobased polymers. Session panelists will highlight recent innovations in plastics and packaging materials. Attendees will also hear how these game-changing biobased materials compete in a global market, what sustainable production means to their supply chain and how to overcome barriers to commercializing these products. 105 Minutes Plenary Richard P. Eno - Moderator
Thijs Rodenburg
Michael Saltzberg
Bob Walsh
Puneet Trehan
Gustavo Sergi
Assessment Methods for Newly Launched Bio-Pharma Products (Stage 3A) This article provide a much needed methodology in completing Stage 3A assessment for a newly launched Bio-Pharma products. The comprehensive assessment reviews data from process development stages through scale-up, qualification and commercial manufacturing. The conclusions made should provide sufficient information to make a scientific and risk-based decision on Bio-Pharma Product Robustness and Product Quality. 90 minutes Poster Ajay Babu Pazhayattil
A Thriving Bioeconomy: USDA’s Role in Policy, Research, Development and Commercialization A thriving bioeconomy requires research, commercialization, programs and policy to bring biobased products, made from both agricultural and forests feedstocks, to market. USDA’s efforts include R&D, technology transfer-marketing and verification, commercialization and government procurement programs. USDA maintains the nation’s largest microbial collection used to improve biorefining efficiencies, lower biofuels cost, and create new commercial bio-products. Improving biorefining efficiencies in the future will focus on reduced antimicrobial resistance. USDA’s grant and guaranteed loan programs finance construction and development of commercial scale biorefineries and manufacturing facilities and advancement of new and emerging technologies for the production of advanced biofuels, renewable chemicals, and biobased products. Also discussed will be the USDA BioPreferred® Program’s follow-up study to its 2015 report: An Economic Impact Analysis of the U.S. Biobased Product Industry, which examines the economic effects of the U.S./state industry. The budget, next farm bill and direction from the administration will provide guidance moving forward. 75 Minutes Workshop Gene Lester
Mark Brodziski
Marie Wheat
World Nieh
Harry Baumes - Moderator
BacHBerry: Bacterial Hosts for Production of Bioactive Phenolics from Berry Fruits Bacterial hosts for production of bioactive phenolics from berry fruits (BacHBerry) is a 3 year project initiated in November 2014 that is funded by the Seventh Framework Programme for Research and Technological Development (FP7) of the European Union. Together with DISCO and TriForC the project falls under the theme KBBE.2013.3.1-01: “Plant High Value Products – from discovery to final product”. The consortium includes twelve research groups, five small and medium-sized enterprises, and one large enterprise coming from 10 different countries, including Chile, China, and Russia. The overall goal of BacHBerry is to develop a sustainable and a cost-effective pipeline for production of high-value berry phenolic compounds using microbial platforms. The process is to be designed in a way to allow subsequent commercialization, aiming at providing socio-economic benefits for communities inside and outside of Europe. The project covers the entire scope of discovery and pre-commercialization activities, from germplasm collection screening and identification of novel bioactive polyphenols to functional characterization of the corresponding biosynthetic genes and construction of Gram-positive cell factories, with further optimization of extraction methods and scale-up of the production by fermentation up to pilot scale. At the time of the symposium the project would be completed and the key outcomes of it would be presented herein. 90 minutes Poster Alexey Dudnik
below50: Promoting the World’s Most Sustainable Fuels below50 is a global campaign that seeks to de-carbonize transportation by growing the use of sustainable fuels with CO2 emissions less than 50% of conventional fossil fuels. below50 communicates the availability and benefits of below50 fuels to policymakers, NGOs and corporate fuel buyers. below50 works to convert the global momentum for combatting Climate Change - as embodied in the Paris Agreement - into concrete actions at the National and sub-National level. Specifically, below50 seeks to create novel demand for below50 fuels by creating business-to-business (B2B) opportunities across the below50 fuel value chain. This workshop will be the first public activity of below50 North America. We look forward to meeting with investors, technology providers, fuel producers and, importantly, fuel buyers to discuss ways to increase the use of below50 fuels in North America. 75 Minutes Workshop Aaron Robinson
Larissa Rose
Hans van der Sluijs
Michael Burns
Gerard Ostheimer - Moderator
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